Combining in-cell NMR and X-ray fluorescence microscopy to reveal the intracellular maturation states of human superoxide dismutase 1.

نویسندگان

  • E Luchinat
  • A Gianoncelli
  • T Mello
  • A Galli
  • L Banci
چکیده

An integrated approach which combines in-cell NMR spectroscopy with optical and X-ray fluorescence microscopy was developed to describe the intracellular maturation state of human Cu,Zn-SOD1. Microscopy data show a correlation between the intracellular levels of SOD1 and the content of zinc, corresponding to zinc binding to SOD1 observed by in-cell NMR.

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عنوان ژورنال:
  • Chemical communications

دوره 51 3  شماره 

صفحات  -

تاریخ انتشار 2015